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Tuesday, July 14, 2020 | History

2 edition of Electrogenic H+ transport and its relationship to the NADPH oxidase of phagocytic cells. found in the catalog.

Electrogenic H+ transport and its relationship to the NADPH oxidase of phagocytic cells.

Arvind Nanda

Electrogenic H+ transport and its relationship to the NADPH oxidase of phagocytic cells.

by Arvind Nanda

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Published .
Written in English


The Physical Object
Pagination255 leaves.
Number of Pages255
ID Numbers
Open LibraryOL15463724M
ISBN 100612118150

The H+ channel associated with the generation of O by NADPH oxidase and the oxidase itself must both be activated in response to stimuli (e.g. phorbol esters, chemotactic peptides, certain. (). The superoxide-generating NADPH oxidase of human neutrophils is electrogenic and associated with an H+ channel. (). The voltage dependence of NADPH oxidase reveals why phagocytes need proton channels. (). V-ATPases in phagocytic cells. (). Voltage-gated proton channel is expressed on phagosomes. ().

Electron transfer through the NADPH oxidase is electrogenic and is associated with a depolarization of the plasma membr The magnitude of this depolarization is unknown but likely exceeds the threshold of activation of the phagocytic H + conductance, since conductive H + efflux has been reported during the respiratory burst Methods are included for testing assembly and function of multicomponent oxidase complexes and for analyzing reactive oxygen species (ROS) generation in different systems by various means, while addressing pitfalls of ROS probes currently being used, as well as protocols on NADPH oxidase regulation and their function in cells.

The NADPH Oxidase and Microbial Killing by Neutrophils, With a Particular Emphasis on the Proposed Antimicrobial Role of Myeloperoxidase within the Phagocytic Vacuole, p In Gordon S (ed), Myeloid Cells in Health and Disease. ASM Press, Washington, . El-Benna, J. Regulation of the phagocyte NADPH oxidase activity: phosphorylation of gp91phox/NOX2 by protein kinase C enhances its diaphorase activity and binding to Rac2, p67 phox, and p FASEB J. 23, – () Key Words: neutrophils PMN cytochrome b inflam-mation innate immunity Professional phagocytic cells play a central role in.


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Electrogenic H+ transport and its relationship to the NADPH oxidase of phagocytic cells by Arvind Nanda Download PDF EPUB FB2

In the neutrophil, electron transport through the NADPH oxidase of phagocytic cells depolarizes the membrane of the phagocytic vacuole and acidifies the cytosol, whereas the generation of O 2 − in the vacuole alkalinizes this compartment.

These changes induce secondary ion fluxes, which result in activation of the granule proteins discharged Cited by:   In view of all these ion movements initiated by the charge separation via the NADPH oxidase, we had proposed that alteration of the ionic composition of the intraphagosomal space may contribute to the impairment of bacterial killing in CGD.

17 Recently Reeves and coworkers questioned the toxic effect of various ROS under the conditions of the phagosome. 18 Instead, they suggested a Cited by:   NADPH oxidase of phagocytic cells transfers a single electron from intracellular NADPH to extracellular O 2, producing superoxide (O 2 −), the precursor to several other reactive oxygen finding that a genetic defect of the enzyme causes chronic granulomatous disease (CGD), characterized by recurrent severe bacterial infections, linked O 2 − generation to destruction Cited by:   To test the suggested structural relationship between the electrogenic H + transporting system and the NADPH oxidase of phagocytes, the existence of the enzyme and the transport process was investigated in human tonsillar T lymphocytes.

It is shown that tonsillar T cells possess an arachidonic acid activatable, Cd 2+ - and Zn 2+-sensitive electrogenic H + efflux pathway with similar Cited by: NADPH oxidase of phagocytic cells transfers a single electron from intracellular NADPH to extracellular O2, producing superoxide (O), the precursor to several other reactive oxygen species.

The NADPH oxidase is an electron transport chain in “professional” phagocytic cells that transfers electrons from NADPH in the cytoplasm, across the wall of the phagocytic vacuole, to form superoxide.

The electron transporting flavocytochrome b is activated by the integrated function of four cytoplasmic proteins. As pig peripheral lymphocytes are not able to produce O, it is suggested that the operation of the electrogenic H+ conductance does not require the assembly of a functional NADPH oxidase.

The NADPH oxidase Nox3 constitutively produces superoxide in a p22phox-dependent manner: its regulation by oxidase organizers and activators. J Biol Chem ; – CAS Google Scholar. As predicted from its electrogenic nature, the transport of electrons by the phox is voltage‐dependent and the amplitude of I e decreases at depolarized voltages.

34 This voltage‐dependence is also observed within the physiological range of membrane potentials provided that enough substrate (i.e., NADPH) is available to fuel the oxidase. Introduction. NADPH oxidases (NOX) are a growing family of plasma membrane enzymes whose function is to generate superoxide by transferring electrons from cytosolic NADPH to extracellular O founding father of the NOX family is the phagocytic NADPH oxidases (phox), which is expressed in phagocytic white blood cells such as neutrophils, eosinophils and.

Summary. The NADPH oxidase producing-superoxide is the major mechanism by which phagocytes kill invading pathogens. The human myeloid cell line PLB was transfected to express p85 cytosolic phospholipase A 2 (cPLA 2) antisense mRNA and stable clones were selected which lack detectable cPLA 2.

cPLA2-deficient PLB cells differentiate similarly to control PLB cells in response to. THE NADPH OXIDASES are a group of plasma membrane–associated enzymes found in a variety of cells of mesodermal most thoroughly studied of these is the leukocyte NADPH oxidase, which is found in professional phagocytes and B lymphocytes.

It catalyzes the production of superoxide (O 2 −) by the one-electron reduction of oxygen, using NADPH as the electron donor. NADPH oxidases (NOX) are a growing family of plasma membrane enzymes whose function is to generate superoxide by transferring electrons from cytosolic NADPH to extracellular O founding father of the NOX family is the phagocytic NADPH oxidases (phox), which is expressed in phagocytic white blood cells such as neutrophils, eosinophils and macrophages (Babior et al.

; Quinn &. The NADPH (nicotinamide adenine dinucleotide phosphate) oxidase (NOX) of 'professional' phagocytic cells transfers electrons across the wall of the phagocytic vacuole, forming superoxide in the lumen. Here we show that COS-7 cells transfected with four NADPH oxidase components, but lacking H+ channels, produce O2- in the presence of Zn2+ concentrations that inhibit O2.

The electron transport chain of the microbicidal oxidase of phagocytic cells and its involvement in the molecular pathology of chronic granulomatous disease. A W Segal Department of Medicine, Faculty of Clinical Science, University College London, United Kingdom.

Rossi F. The O2- -forming NADPH oxidase of the phagocytes: nature, mechanisms of activation and function. Biochim Biophys Acta. Nov 4; (1)– Segal AW. The electron transport chain of the microbicidal oxidase of phagocytic cells and its involvement in the molecular pathology of chronic granulomatous disease.

J Clin Invest. Components of the NADPH oxidase include the flavocytochrome b electron transport chain and cytosolic factors. The electron transporting component is a flavocytochrome that is activated in the wall of the phagocytic vacuole. It is a α 1 β 1 heterodimer. The NADPH oxidase generates superoxide in the professional phagocytic cells, neutrophils.

NADPH oxidase activity is responsible for the killing of intracellular pathogens. Glucose is known to stimulate NADPH oxidase of phagocytic cells. As such, the induction of NADPH oxidase activity within phagocytic cells in diabetic subjects would be expected to enhance the destruction of intracellular pathogens such as Mycobacterium.

Granulocytes generate a “respiratory burst” of NADPH oxidase-dependent superoxide anion (O2−∙) production that is required for efficient clearance of bacterial pathogens. Hv1 mediates a voltage-gated H+ channel activity that is proposed to serve a charge-balancing role in granulocytic phagocytes such as neutrophils and eosinophils.

Using mice in which the gene encoding Hv1 is. NADPH oxidase (nicotinamide adenine dinucleotide phosphate oxidase) is a membrane-bound enzyme complex that faces the extracellular space. It can be found in the plasma membrane as well as in the membranes of phagosomes used by neutrophil white blood cells to engulf microorganisms.

Human isoforms of the catalytic component of the complex include NOX1, NOX2, NOX3, NOX4, NOX5. Phagocytic cells of CG Journal of Leukocyte Biology considers manuscripts of original investigations focusing on the origins, developmental biology, biochemistry and functions of granulocytes, lymphocytes, mononuclear phagocytes, and other cells involved in host defense.

These include full-length papers on original research, rapid.Phagosomal ROS and Ion Transport. NADPH oxidase activity is electrogenic and requires a compensating charge for optimal activity. Lack of a compensating charge can cause extreme voltages (up to + mV) leading to self‐inhibit In addition to protein trafficking, ion transport across the phagosomal membrane has been increasingly.